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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has become noticed in lots of tumors, which results in chromosome instability, and thus could lead to tumorigenesis. This protein was discovered to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a important role in endorsing mobile-cycle development during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, happen to be described. Two extra splice variants were reported but thorough nucleotide sequence information and facts will not be nevertheless obtainable.

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Blocking peptides are peptides that bind precisely towards the focus on antibody and block antibody binding. These peptide ordinarily incorporates the epitope recognized by the antibody.

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This guide outlines even more possible optimization of mobile and immunoassay parameters to ensure the very best outcomes are...

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This manual outlines more possible optimization of mobile and immunoassay parameters to be certain the absolute best results are...

Antibodies sure to the blocking peptide no longer bind for the epitope around the concentrate on protein. By evaluating the staining through the blocked antibody compared to the antibody alone, one can see which staining is particular.

Target Information Cyclin E belongs into the really conserved cyclin household, whose members are characterized by a spectacular periodicity in protein abundance throughout the mobile cycle. Cyclins purpose as regulators of CDK kinases. Distinct cyclins show unique expression and degradation styles which contribute into the temporal coordination of every mitotic party. Cyclin E types a posh with and features for a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S transition. This protein accumulates for the G1-S phase boundary which is degraded as cells development by means of S section.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...

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